6WPC
Crystal structure of Bacillus thuringiensis Cry1A.2 tryptic core variant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-03-20 |
Detector | MARRESEARCH |
Wavelength(s) | 1.000 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 120.578, 225.820, 239.945 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.120 - 2.990 |
R-factor | 0.2008 |
Rwork | 0.198 |
R-free | 0.25790 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4arx |
RMSD bond length | 0.012 |
RMSD bond angle | 1.556 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 120.000 | 3.050 |
High resolution limit [Å] | 2.990 | 3.000 |
Rmerge | 0.182 | 0.483 |
Number of reflections | 62795 | 1992 |
<I/σ(I)> | 7.4 | 1.33 |
Completeness [%] | 95.1 | 60.8 |
Redundancy | 3.47 | 1.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | Wizard 12 screen H7 (10% PEG8000, 0.1 M Tris, pH 7.0, 0.2 M magnesium chloride) |