6WP8
Proton-pumping mutant of Mastigocladopsis repens rhodopsin chloride pump
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-11-17 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.033 |
Spacegroup name | P 3 2 1 |
Unit cell lengths | 103.741, 103.741, 55.213 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.920 - 2.500 |
R-factor | 0.23 |
Rwork | 0.228 |
R-free | 0.24710 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6NWF |
RMSD bond length | 0.004 |
RMSD bond angle | 0.661 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.920 | 2.600 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 11125 | 1275 |
<I/σ(I)> | 3.3 | 1 |
Completeness [%] | 92.0 | 94.6 |
Redundancy | 3.4 | 3.3 |
CC(1/2) | 0.980 | 0.300 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4 | 307.15 | 3.6 M sodium phosphate monobasic monohydrate, pH 4.0, 180 mM 1,6 hexanediol, 3.5% triethylene glycol |