6WP0
The Crystal Structure of Domain-Swapped Trimer Q108K:T51D variant of HCRBPII
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-02-15 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 84.565, 111.272, 101.826 |
| Unit cell angles | 90.00, 112.89, 90.00 |
Refinement procedure
| Resolution | 46.900 - 2.780 |
| R-factor | 0.2777 |
| Rwork | 0.276 |
| R-free | 0.32050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2rct |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.701 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.900 | 2.879 |
| High resolution limit [Å] | 2.780 | 2.780 |
| Rmerge | 0.098 | 0.687 |
| Number of reflections | 43694 | 4222 |
| <I/σ(I)> | 22.5 | 2.5 |
| Completeness [%] | 99.3 | 96.99 |
| Redundancy | 7.5 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | sodium acetate, ammonium acetate, PEG 4000 |
