6WOZ

Plasmodium vivax reticulocyte binding protein 2b (PvRBP2b) bound to human monoclonal antibody 251249

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-17
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 1 21 1
Unit cell lengths	99.568, 163.551, 121.757
Unit cell angles	90.00, 99.19, 90.00

Refinement procedure

Resolution	49.145 - 2.900
R-factor	0.2135
Rwork	0.211
R-free	0.25770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5w53
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.15_3459)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.145	49.145	3.070
High resolution limit [Å]	2.890	8.560	2.890
Rmerge	0.181	0.035	1.370
Rmeas	0.195	0.037	1.484
Total number of observations	603673
Number of reflections	85210	3373	13322
<I/σ(I)>	9.78	41.07	1.33
Completeness [%]	99.4	99.3	96.6
Redundancy	7.085	6.971	6.716
CC(1/2)	0.997	0.999	0.653

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	295	0.2 M DL-malate-imidazole pH 6.0, 15% (w/v) PEG 4,000