6WO1

Hybrid acetohydroxyacid synthase complex structure with Cryptococcus neoformans AHAS catalytic subunit and Saccharomyces cerevisiae AHAS regulatory subunit

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2019-03-28
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.95370
Spacegroup name	P 4 3 2
Unit cell lengths	195.679, 195.679, 195.679
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.920 - 3.300
R-factor	0.2159
Rwork	0.210
R-free	0.26920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ims 2fgc
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.920	3.560
High resolution limit [Å]	3.300	3.300
Rpim	0.041	0.286
Number of reflections	19890	3985
<I/σ(I)>	13.7
Completeness [%]	99.8
Redundancy	19.7
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	Potassium thiocyanate, PEG 3350