6WO0
human Artemis/SNM1C catalytic domain, crystal form 1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-03-26 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 72.690, 111.010, 55.160 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.541 - 1.970 |
Rwork | 0.218 |
R-free | 0.26950 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Model from a SAD data |
RMSD bond length | 0.007 |
RMSD bond angle | 1.499 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 2.020 |
High resolution limit [Å] | 1.970 | 1.970 |
Rmerge | 0.050 | 0.828 |
Number of reflections | 32168 | 2318 |
<I/σ(I)> | 16 | |
Completeness [%] | 99.6 | |
Redundancy | 6.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 300 | 50 mM MES pH 6.5, 0.1 M LiCl, 0.01 M MgCl2, 12% PEG 4000 (w/v) |