6WNL

human Artemis/SNM1C catalytic domain, crystal form 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-24
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.0333
Spacegroup name	P 21 21 21
Unit cell lengths	69.209, 105.675, 116.037
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.462 - 2.370
Rwork	0.221
R-free	0.28030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	SAD model
RMSD bond length	0.004
RMSD bond angle	1.330
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.430
High resolution limit [Å]	2.370	2.370
Rmerge	0.148	0.784
Number of reflections	35315	2574
<I/σ(I)>	9.7
Completeness [%]	100.0
Redundancy	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	300	50 mM MES pH 6.5, 0.1 M LiCl, 0.01 M MgCl2, 12% PEG 4000 (w/v)