6WMI
ZNF410 zinc fingers 1-5 with 17 mer blunt DNA Oligonucleotide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-03-10 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.00000 |
Spacegroup name | P 62 |
Unit cell lengths | 186.257, 186.257, 46.950 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 41.920 - 2.750 |
R-factor | 0.1609 |
Rwork | 0.159 |
R-free | 0.19410 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 0.683 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.920 | 2.850 |
High resolution limit [Å] | 2.750 | 2.750 |
Rmerge | 0.200 | 0.500 |
Rpim | 0.070 | 0.820 |
Number of reflections | 47241 | 4697 |
<I/σ(I)> | 12.3 | 1.3 |
Completeness [%] | 99.9 | 100 |
Redundancy | 11.1 | 10 |
CC(1/2) | 0.991 | 0.498 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 292 | 0.2 M ammonium formate, 20% PEG3350 |