6WMF

Human Sun2-KASH5 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-07-31
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9792
Spacegroup name	H 3 2
Unit cell lengths	78.435, 78.435, 249.441
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	83.150 - 2.600
R-factor	0.2278
Rwork	0.221
R-free	0.28590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4dxt
RMSD bond length	0.010
RMSD bond angle	1.213
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.17_3644)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	83.150	2.740
High resolution limit [Å]	2.600	2.600
Rpim	0.025	0.466
Number of reflections	9383	1257
<I/σ(I)>	37.3	0.9
Completeness [%]	99.2	95
Redundancy	15.9	6.3
CC(1/2)	0.999	0.510

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	14% PEG3000, 0.1M BisTris/HCl