6WMC
Crystal structure of a soluble variant of full-length human APOBEC3G (pH 9.0)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-02-08 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 1 |
Spacegroup name | I 1 2 1 |
Unit cell lengths | 60.233, 42.091, 191.911 |
Unit cell angles | 90.00, 95.94, 90.00 |
Refinement procedure
Resolution | 47.720 - 3.490 |
R-factor | 0.2222 |
Rwork | 0.216 |
R-free | 0.27420 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6BUX CHAIN A AND 5K81 CHAIN A |
RMSD bond length | 0.002 |
RMSD bond angle | 0.508 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.630 |
High resolution limit [Å] | 3.490 | 3.490 |
Rmerge | 0.158 | 0.497 |
Number of reflections | 5588 | 482 |
<I/σ(I)> | 5.08 | 1.37 |
Completeness [%] | 89.0 | 83.4 |
Redundancy | 2.8 | 2.6 |
CC(1/2) | 0.985 | 0.718 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9 | 277 | 0.1M BICINE (pH 9.0) and 10% w/v PEG 6000 |