Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-03 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 31 |
| Unit cell lengths | 126.270, 126.270, 94.850 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 63.130 - 2.600 |
| R-factor | 0.2124 |
| Rwork | 0.207 |
| R-free | 0.25810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | fixed/fixed.pdb |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_3724) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 63.130 | 63.130 | 2.670 |
| High resolution limit [Å] | 2.600 | 11.620 | 2.600 |
| Rmerge | 0.129 | 0.034 | 1.277 |
| Rmeas | 0.143 | 0.038 | 1.416 |
| Total number of observations | 274668 | ||
| Number of reflections | 51879 | 583 | 3776 |
| <I/σ(I)> | 11.31 | 35.99 | 1.5 |
| Completeness [%] | 99.6 | 99.8 | 98.2 |
| Redundancy | 5.294 | 5.36 | 5.279 |
| CC(1/2) | 0.996 | 0.999 | 0.539 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 298 | 1M Lithium chloride, 0.1 Citric acid pH 4.0, 20% (w/v) PEG 6000 |
