6WL5

Crystal structure of EcmrR C-terminal domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-07
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97920
Spacegroup name	C 1 2 1
Unit cell lengths	212.393, 42.419, 115.798
Unit cell angles	90.00, 117.27, 90.00

Refinement procedure

Resolution	102.930 - 1.400
R-factor	0.1435
Rwork	0.142
R-free	0.17280
Structure solution method	MIRAS
RMSD bond length	0.014
RMSD bond angle	1.468
Data reduction software	XDS
Data scaling software	XDS
Phasing software	AutoSol
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	103.210	1.450
High resolution limit [Å]	1.400	1.400
Rmerge	0.049	0.601
Rmeas	0.059	0.715
Rpim	0.032	0.382
Number of reflections	178379	17848
<I/σ(I)>	12.4	1.7
Completeness [%]	98.4	99.2
Redundancy	3.4	3.4
CC(1/2)	0.999	0.707

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.5 M sodium chloride, 0.01 M magnesium chloride, 0.01 M cetyltrimethylammonium bromide