6WL5
Crystal structure of EcmrR C-terminal domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97920 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 212.393, 42.419, 115.798 |
| Unit cell angles | 90.00, 117.27, 90.00 |
Refinement procedure
| Resolution | 102.930 - 1.400 |
| R-factor | 0.1435 |
| Rwork | 0.142 |
| R-free | 0.17280 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.468 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 103.210 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.049 | 0.601 |
| Rmeas | 0.059 | 0.715 |
| Rpim | 0.032 | 0.382 |
| Number of reflections | 178379 | 17848 |
| <I/σ(I)> | 12.4 | 1.7 |
| Completeness [%] | 98.4 | 99.2 |
| Redundancy | 3.4 | 3.4 |
| CC(1/2) | 0.999 | 0.707 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.5 M sodium chloride, 0.01 M magnesium chloride, 0.01 M cetyltrimethylammonium bromide |
