6WKL

Fab Fragment of Anti-human LAG3 antibody (BAP050)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-02
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9796
Spacegroup name	P 21 21 21
Unit cell lengths	67.770, 75.530, 103.060
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.310 - 1.890
R-factor	0.1947
Rwork	0.193
R-free	0.22340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1mim
RMSD bond length	0.007
RMSD bond angle	0.891
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.310	1.940
High resolution limit [Å]	1.890	1.890
Rmerge	0.100	0.900
Rmeas	0.130
Rpim	0.095	0.890
Number of reflections	41427	2239
<I/σ(I)>	5.9	1
Completeness [%]	97.0	82.5
Redundancy	3	2.2
CC(1/2)	0.980	0.230

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	296	PEG 8000, HEPES Na