6WK5
Crystal structure of Gdx-Clo from Small Multidrug Resistance family of transporters
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2019-10-12 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.9786 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 141.800, 51.093, 108.433 |
Unit cell angles | 90.00, 93.08, 90.00 |
Refinement procedure
Resolution | 44.230 - 3.500 |
R-factor | 0.2534 |
Rwork | 0.252 |
R-free | 0.28580 |
Structure solution method | SAD |
Data reduction software | DIALS |
Data scaling software | Aimless |
Phasing software | SHARP |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 54.140 | 3.830 |
High resolution limit [Å] | 3.500 | 3.500 |
Rmerge | 0.060 | 0.148 |
Rmeas | 0.070 | 0.171 |
Rpim | 0.035 | 0.086 |
Number of reflections | 10080 | 2389 |
<I/σ(I)> | 17.3 | |
Completeness [%] | 100.0 | |
Redundancy | 7.5 | |
CC(1/2) | 0.995 | 0.995 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.8 | 294 | 0.1 M LiNO3, 0.1 M N-(2-Acetamido)iminodiacetic acid (ADA) pH 6.8, and 35% PEG 600 |