6WJO
Crystal structure of wild-type Arginine Repressor from the pathogenic bacterium Corynebacterium pseudotuberculosis bound to tyrosine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LNLS BEAMLINE W01B-MX2 |
Synchrotron site | LNLS |
Beamline | W01B-MX2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-08-17 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1.4586 |
Spacegroup name | P 21 3 |
Unit cell lengths | 82.990, 82.990, 82.990 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.748 - 1.693 |
R-factor | 0.1859 |
Rwork | 0.181 |
R-free | 0.21150 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5jvo |
RMSD bond length | 0.007 |
RMSD bond angle | 0.901 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.220 | 1.800 |
High resolution limit [Å] | 1.690 | 1.690 |
Rmerge | 0.071 | 1.131 |
Rmeas | 0.074 | 1.089 |
Number of reflections | 21903 | 3488 |
<I/σ(I)> | 22.97 | 1.74 |
Completeness [%] | 99.4 | 97.1 |
Redundancy | 11.8 | 9.1 |
CC(1/2) | 0.999 | 0.583 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291.15 | 2.0 M Sodium Formate, pH 7.5 |