6WIZ

Crystal structure of Fab 54-1G05 bound to H1 influenza hemagglutinin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-10
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0332
Spacegroup name	P 3 2 1
Unit cell lengths	187.378, 187.378, 132.533
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.844 - 4.200
R-factor	0.3258
Rwork	0.323
R-free	0.38920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6fyt
Data scaling software	Aimless (0.7.2)
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.844	46.840	4.600
High resolution limit [Å]	4.200	10.290	4.200
Rmerge	0.401	0.140	0.990
Rmeas	0.412	0.143	1.017
Rpim	0.093	0.033	0.232
Total number of observations		26826	89611
Number of reflections	19952	1442	4703
<I/σ(I)>	4.8	9.8	2.6
Completeness [%]	99.9	99	100
Redundancy	19.2	18.6	19.1
CC(1/2)	0.994	0.996	0.970

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	50% PEG 200 and 0.1 M CHES pH 9.5