6WIZ
Crystal structure of Fab 54-1G05 bound to H1 influenza hemagglutinin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-10-10 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.0332 |
Spacegroup name | P 3 2 1 |
Unit cell lengths | 187.378, 187.378, 132.533 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.844 - 4.200 |
R-factor | 0.3258 |
Rwork | 0.323 |
R-free | 0.38920 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6fyt |
Data scaling software | Aimless (0.7.2) |
Phasing software | PHASER |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 46.844 | 46.840 | 4.600 |
High resolution limit [Å] | 4.200 | 10.290 | 4.200 |
Rmerge | 0.401 | 0.140 | 0.990 |
Rmeas | 0.412 | 0.143 | 1.017 |
Rpim | 0.093 | 0.033 | 0.232 |
Total number of observations | 26826 | 89611 | |
Number of reflections | 19952 | 1442 | 4703 |
<I/σ(I)> | 4.8 | 9.8 | 2.6 |
Completeness [%] | 99.9 | 99 | 100 |
Redundancy | 19.2 | 18.6 | 19.1 |
CC(1/2) | 0.994 | 0.996 | 0.970 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 50% PEG 200 and 0.1 M CHES pH 9.5 |