6WIS

Copper resistance protein copG- Form 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-14
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9792
Spacegroup name	P 61
Unit cell lengths	86.130, 86.130, 58.560
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.061 - 2.000
R-factor	0.177
Rwork	0.172
R-free	0.22040
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	AutoSol
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	74.600	2.050
High resolution limit [Å]	2.000	2.000
Rmerge	0.133	0.928
Number of reflections	32888	2437
<I/σ(I)>	10.7
Completeness [%]	99.9	99.8
Redundancy	7.1	7.4
CC(1/2)	0.996	0.594

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	100 mM Na Cacodylate 200 mM Zn Acetate 17% ethanol