6WIR
Fab antigen complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-06-25 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 73.400, 73.400, 326.409 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 71.610 - 2.960 |
R-factor | 0.219 |
Rwork | 0.217 |
R-free | 0.26000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5vb9 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.240 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | BUSTER (2.11.7) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 71.610 | 3.120 |
High resolution limit [Å] | 2.960 | 2.960 |
Rmerge | 0.080 | |
Rpim | 0.242 | |
Number of reflections | 19713 | 2781 |
<I/σ(I)> | 20.2 | 2.9 |
Completeness [%] | 100.0 | |
Redundancy | 12.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 274 | 100mM Hepes pH 7, 15% PEG4K, 200mM Magnesium Chloride |