6WIN

Type 6 secretion amidase effector 2 (Tae2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	91
Detector technology	PIXEL
Collection date	2017-12-22
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97954
Spacegroup name	P 1 21 1
Unit cell lengths	33.318, 46.032, 38.923
Unit cell angles	90.00, 104.71, 90.00

Refinement procedure

Resolution	37.650 - 2.050
R-factor	0.1795
Rwork	0.174
R-free	0.22380
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	0.811
Data reduction software	ELVES
Data scaling software	ELVES
Phasing software	AutoSol
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.030	1.710
High resolution limit [Å]	1.680	1.680
Rmerge	0.089	0.989
Rmeas	0.098	1.255
Rpim	0.039	0.757
Total number of observations	52864	84
Number of reflections	9246	37
<I/σ(I)>	16	1.4
Completeness [%]	70.4	5.7
Redundancy	5.7	2.3
CC(1/2)	0.997	0.443

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	1M sodium citrate 0.1M MES pH 6.6 4% formamide