6WIN
Type 6 secretion amidase effector 2 (Tae2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 91 |
Detector technology | PIXEL |
Collection date | 2017-12-22 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97954 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 33.318, 46.032, 38.923 |
Unit cell angles | 90.00, 104.71, 90.00 |
Refinement procedure
Resolution | 37.650 - 2.050 |
R-factor | 0.1795 |
Rwork | 0.174 |
R-free | 0.22380 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 0.811 |
Data reduction software | ELVES |
Data scaling software | ELVES |
Phasing software | AutoSol |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.030 | 1.710 |
High resolution limit [Å] | 1.680 | 1.680 |
Rmerge | 0.089 | 0.989 |
Rmeas | 0.098 | 1.255 |
Rpim | 0.039 | 0.757 |
Total number of observations | 52864 | 84 |
Number of reflections | 9246 | 37 |
<I/σ(I)> | 16 | 1.4 |
Completeness [%] | 70.4 | 5.7 |
Redundancy | 5.7 | 2.3 |
CC(1/2) | 0.997 | 0.443 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 1M sodium citrate 0.1M MES pH 6.6 4% formamide |