6WIN
Type 6 secretion amidase effector 2 (Tae2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 91 |
| Detector technology | PIXEL |
| Collection date | 2017-12-22 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97954 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 33.318, 46.032, 38.923 |
| Unit cell angles | 90.00, 104.71, 90.00 |
Refinement procedure
| Resolution | 37.650 - 2.050 |
| R-factor | 0.1795 |
| Rwork | 0.174 |
| R-free | 0.22380 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.811 |
| Data reduction software | ELVES |
| Data scaling software | ELVES |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.030 | 1.710 |
| High resolution limit [Å] | 1.680 | 1.680 |
| Rmerge | 0.089 | 0.989 |
| Rmeas | 0.098 | 1.255 |
| Rpim | 0.039 | 0.757 |
| Total number of observations | 52864 | 84 |
| Number of reflections | 9246 | 37 |
| <I/σ(I)> | 16 | 1.4 |
| Completeness [%] | 70.4 | 5.7 |
| Redundancy | 5.7 | 2.3 |
| CC(1/2) | 0.997 | 0.443 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 1M sodium citrate 0.1M MES pH 6.6 4% formamide |
