6WIG
Structure of STENOFOLIA Protein HD domain bound with DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-08-03 |
Detector | DECTRIS PILATUS3 X 6M |
Wavelength(s) | 0.97935 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 48.061, 49.489, 69.810 |
Unit cell angles | 90.00, 106.46, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.100 |
R-factor | 0.1997 |
Rwork | 0.194 |
R-free | 0.24980 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.214 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.080 | 0.662 |
Number of reflections | 17541 | 1431 |
<I/σ(I)> | 4.4 | |
Completeness [%] | 95.2 | 77.7 |
Redundancy | 8.1 | |
CC(1/2) | 0.814 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | .15 M sodium chloride 28 % v/v PEG Smear Medium |