6WHB

MEKK1 TOG domain (548-867)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-02-01
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.9537
Spacegroup name	I 41 2 2
Unit cell lengths	71.817, 71.817, 259.561
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.291 - 1.900
R-factor	0.208269068866
Rwork	0.207
R-free	0.22969
Structure solution method	SIRAS
RMSD bond length	0.006
RMSD bond angle	0.728
Data reduction software	XDS
Data scaling software	Aimless (0.5.32)
Phasing software	Auto-Rickshaw
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.300	1.968
High resolution limit [Å]	1.900	1.900
Rmerge	0.145
Rmeas	0.147
Number of reflections	27403	2683
<I/σ(I)>	19.36
Completeness [%]	99.0	100
Redundancy	28.6	29
CC(1/2)	1.000	0.239

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.9	290.15	0.1 M Bis-Tris Propane pH 6.9, 1.5 M sodium acetate
1	VAPOR DIFFUSION, SITTING DROP	6.9	290.15	0.1 M Bis-Tris Propane pH 6.9, 1.5 M sodium acetate