6WGZ

Crystal structure of HyBcl-2-4 with HyBak1 BH3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-03-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	54.245, 73.650, 140.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.680 - 2.200
R-factor	0.2238
Rwork	0.222
R-free	0.25110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1pq1
RMSD bond length	0.003
RMSD bond angle	0.624
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.680	2.270
High resolution limit [Å]	2.190	2.190
Rmerge	0.137	1.560
Number of reflections	29115	2769
<I/σ(I)>	8.6	1.4
Completeness [%]	98.5
Redundancy	10.8
CC(1/2)	0.990	0.940

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.8	293	0.2M Magnesium acetate tetra hydrate, 20% PEG 3350