6WF0
Crystal Structure of Broadly Neutralizing Antibody 3I14 Bound to the Influenza A H3 Hemagglutinin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 293 |
Detector technology | CCD |
Collection date | 2017-04-17 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | .97919 |
Spacegroup name | P 63 |
Unit cell lengths | 130.318, 130.318, 188.552 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.660 - 3.460 |
R-factor | 0.2353 |
Rwork | 0.231 |
R-free | 0.28190 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 405I |
RMSD bond length | 0.003 |
RMSD bond angle | 0.652 |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.660 | 3.581 |
High resolution limit [Å] | 3.457 | 3.457 |
Rmerge | 0.196 | 0.992 |
Rmeas | 0.216 | 1.095 |
Rpim | 0.090 | 0.459 |
Number of reflections | 23612 | 2349 |
<I/σ(I)> | 8.92 | 1.74 |
Completeness [%] | 99.1 | 98.7 |
Redundancy | 5.5 | |
CC(1/2) | 0.989 | 0.390 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 18-24% w/v PEG 3350, 0.2M KCN, and 0.1M Bis-Tris-Propane pH7.5 |