6WEF
Copper-bound D92H variant of Campylobacter jejuni P19
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-09-21 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 32 |
| Unit cell lengths | 132.455, 132.455, 104.585 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.677 - 2.501 |
| R-factor | 0.2716 |
| Rwork | 0.270 |
| R-free | 0.29910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3lzl |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER (2.8.1) |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 6.780 | 2.500 |
| Rmerge | 0.109 | 0.029 | 0.624 |
| Rmeas | 0.121 | 0.032 | 0.735 |
| Rpim | 0.054 | 0.013 | 0.381 |
| Total number of observations | 341533 | ||
| Number of reflections | 70171 | 3515 | 3406 |
| <I/σ(I)> | 4.6 | ||
| Completeness [%] | 99.0 | 98.9 | 97.3 |
| Redundancy | 4.9 | 5.6 | 3.5 |
| CC(1/2) | 1.000 | 0.900 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 2.4 M ammonium sulfate, 0.2 M lithium sulfate and 0.1 M Tris pH 7 |
