6WCY
N160D Deamidation Mutant of Human gammaD-Crystallin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-12-05 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.811, 51.872, 113.991 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.590 - 1.204 |
| R-factor | 0.1701 |
| Rwork | 0.169 |
| R-free | 0.19440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hk0 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.208 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER (2.8.3) |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.590 | 1.247 |
| High resolution limit [Å] | 1.204 | 1.204 |
| Rmerge | 0.053 | 1.185 |
| Rmeas | 0.055 | |
| Rpim | 0.015 | |
| Number of reflections | 85435 | 4861 |
| <I/σ(I)> | 22.5 | 1 |
| Completeness [%] | 90.9 | 52.45 |
| Redundancy | 12.9 | |
| CC(1/2) | 0.999 | 0.450 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.1 M succinic acid (pH 7.0), 15% w/v PEG 3350 |
