6WCU
Crystal structure of coiled coil region of human septin 5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-09-26 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9795 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 22.593, 26.114, 45.468 |
Unit cell angles | 90.00, 104.28, 90.00 |
Refinement procedure
Resolution | 22.465 - 1.800 |
R-factor | 0.2267 |
Rwork | 0.219 |
R-free | 0.24320 |
Structure solution method | AB INITIO PHASING |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Phasing software | Arcimboldo |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 26.110 | 26.110 | 1.840 |
High resolution limit [Å] | 1.800 | 8.630 | 1.800 |
Rmerge | 0.060 | 0.050 | 0.213 |
Rmeas | 0.073 | 0.062 | 0.259 |
Rpim | 0.040 | 0.035 | 0.145 |
Total number of observations | 14437 | 105 | 950 |
Number of reflections | 4769 | 40 | 306 |
<I/σ(I)> | 11.3 | 16.7 | 4.5 |
Completeness [%] | 97.4 | 82.9 | 97.1 |
Redundancy | 3 | 2.6 | 3.1 |
CC(1/2) | 0.995 | 0.997 | 0.977 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1 M citric acid pH 5.0, 1.6 M ammonium sulfate |