6WCS
Crystal structure of Arabidopsis thaliana isochorismoyl-glutamate A pyruvoyl-glutamate lyase in complex with tartrate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-02-16 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.352, 79.432, 194.214 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 73.520 - 1.870 |
R-factor | 0.2036 |
Rwork | 0.203 |
R-free | 0.22560 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6dd2 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 73.520 | 8.940 |
High resolution limit [Å] | 1.870 | 1.870 |
Rmerge | 0.089 | 1.785 |
Rmeas | 0.094 | 0.032 |
Rpim | 0.026 | 0.507 |
Number of reflections | 69622 | 4240 |
<I/σ(I)> | 16.9 | 1.1 |
Completeness [%] | 99.7 | 95.1 |
Redundancy | 13.1 | 12.6 |
CC(1/2) | 0.999 | 0.661 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 300 | 0.2 M Potassium Sodium Tartrate 20 % (w/v) PEG 3350 |