6WCS

Crystal structure of Arabidopsis thaliana isochorismoyl-glutamate A pyruvoyl-glutamate lyase in complex with tartrate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	53.352, 79.432, 194.214
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	73.520 - 1.870
R-factor	0.2036
Rwork	0.203
R-free	0.22560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6dd2
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	73.520	8.940
High resolution limit [Å]	1.870	1.870
Rmerge	0.089	1.785
Rmeas	0.094	0.032
Rpim	0.026	0.507
Number of reflections	69622	4240
<I/σ(I)>	16.9	1.1
Completeness [%]	99.7	95.1
Redundancy	13.1	12.6
CC(1/2)	0.999	0.661

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		300	0.2 M Potassium Sodium Tartrate 20 % (w/v) PEG 3350