6WCM
Crystal structure of Laccase from Thermus thermophilus HB27 with an open conformation of beta-hairpin (Average deposited dose 15.50 MGy)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-04-17 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.1949 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 54.436, 60.817, 76.235 |
Unit cell angles | 90.00, 109.34, 90.00 |
Refinement procedure
Resolution | 39.240 - 1.700 |
R-factor | 0.1986 |
Rwork | 0.197 |
R-free | 0.23750 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2xu9 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER (7.0.076) |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 39.240 | 38.840 | 1.760 |
High resolution limit [Å] | 1.700 | 3.660 | 1.700 |
Rmerge | 0.050 | 0.027 | 0.653 |
Rmeas | 0.071 | 0.038 | 0.924 |
Rpim | 0.050 | 0.027 | 0.653 |
Number of reflections | 51812 | 5306 | 5144 |
<I/σ(I)> | 7.1 | 16.9 | 1.3 |
Completeness [%] | 100.0 | 100 | 100 |
Redundancy | 1.9 | 1.9 | 1.3 |
CC(1/2) | 0.996 | 0.996 | 0.451 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 5.5 | 277.15 | 20% PEG 4000, 20% 2-propanol, 100 mM citrate buffer |