6WCE
Structure of the periplasmic binding protein P5PA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 105 |
| Detector technology | CCD |
| Collection date | 2014-08-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.468, 42.273, 69.601 |
| Unit cell angles | 90.00, 106.82, 90.00 |
Refinement procedure
| Resolution | 66.620 - 1.754 |
| R-factor | 0.1623 |
| Rwork | 0.161 |
| R-free | 0.19490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4r73 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.786 |
| Data reduction software | iMOSFLM |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.624 | 1.817 |
| High resolution limit [Å] | 1.750 | 1.754 |
| Number of reflections | 28927 | 2856 |
| <I/σ(I)> | 24.68 | |
| Completeness [%] | 92.8 | |
| Redundancy | 5.1 | |
| CC(1/2) | 0.996 | 0.985 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 294.15 | 0.05M potassium thiocyanate, 27.5% PEG MME 2000 |
