6WC3

Crystal structure of the SNARE Sec20 bound to Dsl1 complex subunit Tip20

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-20
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.97930
Spacegroup name	P 62 2 2
Unit cell lengths	133.275, 133.275, 288.470
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.294 - 3.203
R-factor	0.2132
Rwork	0.210
R-free	0.24820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3fhn
Data reduction software	XDS
Data scaling software	Aimless (0.5.31)
Phasing software	PHASER (2.5.5)
Refinement software	PHENIX (1.13.2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.260	29.260	3.280
High resolution limit [Å]	3.200	14.320	3.200
Rmerge	0.132	0.032	1.174
Rmeas	0.135	0.033	1.200
Rpim	0.026	0.007	0.244
Total number of observations	666066	6567	35919
Number of reflections	25597	328	1638
<I/σ(I)>	27.6	90	3.2
Completeness [%]	99.0	88.6	88.4
Redundancy	26	20	21.9
CC(1/2)	1.000	1.000	0.878

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	295	0.1 M sodium citrate, pH 6.0, 0.725 M ammonium sulfate, 1 mM dithiothreitol, cryoprotected with 30% (v/v) glycerol