6WC1

Peptide-bound SARS-CoV-2 Nsp9 RNA-replicase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2020-03-01
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.541
Spacegroup name	P 61 2 2
Unit cell lengths	88.670, 88.670, 134.020
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	76.800 - 2.400
R-factor	0.2344
Rwork	0.232
R-free	0.27800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1qz8
RMSD bond length	0.006
RMSD bond angle	0.944
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	76.800	2.500
High resolution limit [Å]	2.400	2.400
Rpim	0.018	0.291
Number of reflections	12038	1307
<I/σ(I)>	23.4	2.5
Completeness [%]	94.8	73.3
Redundancy	16.2	12.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4	293	2-2.4M AmSO4, 0.1M phosphate citrate pH 4