6WBT
2.52 Angstrom Resolution Crystal Structure of 6-phospho-alpha-glucosidase from Gut Microorganisms in Complex with NAD and Glucose-6-phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-10-19 |
| Detector | DECTRIS PILATUS3 X 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 102.797, 219.724, 97.978 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 70.920 - 2.520 |
| R-factor | 0.229 |
| Rwork | 0.227 |
| R-free | 0.26600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1u8x |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.489 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.090 | 2.560 |
| High resolution limit [Å] | 2.520 | 2.520 |
| Rmerge | 0.121 | 0.978 |
| Number of reflections | 70393 | 3335 |
| <I/σ(I)> | 5 | 1 |
| Completeness [%] | 92.7 | 89.4 |
| Redundancy | 3.4 | 3.4 |
| CC(1/2) | 0.981 | 0.402 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 289 | 0.2 M NaCl, 0.1 M Na/K phosphate pH 6.2, 20 %(w/v) PEG1000 |
