6WBR
Crystal structure of AceCas9 bound with guide RNA and DNA with 5'-NNNCC-3' PAM
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-07-18 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 82.313, 119.348, 177.183 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 74.650 - 2.910 |
R-factor | 0.2262 |
Rwork | 0.224 |
R-free | 0.26610 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.128 |
Data reduction software | XDS |
Data scaling software | AutoProcess |
Phasing software | PHENIX |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 142.600 | 3.000 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.476 | 2.000 |
Rmeas | 0.486 | |
Rpim | 0.099 | 0.781 |
Number of reflections | 55715 | 4272 |
<I/σ(I)> | 6.1 | 0.9 |
Completeness [%] | 98.3 | 97.5 |
Redundancy | 7.2 | 7.4 |
CC(1/2) | 0.976 | 0.362 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 303.15 | 0.04 M Citric acid, 0.06 M Bis-tris propane and 15-20% polyethylene glycol 3350 |