6WBP
Crystal structure of coiled coil region of human septin 6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-01-26 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9200 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 49.640, 49.640, 228.549 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.990 - 1.800 |
| R-factor | 0.2174 |
| Rwork | 0.216 |
| R-free | 0.25470 |
| Structure solution method | AB INITIO PHASING |
| Data reduction software | xia2 |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | Arcimboldo |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 57.140 | 57.140 | 1.840 |
| High resolution limit [Å] | 1.800 | 9.000 | 1.800 |
| Rmerge | 0.065 | 0.034 | 1.147 |
| Rmeas | 0.067 | 0.036 | 1.204 |
| Rpim | 0.017 | 0.010 | 0.361 |
| Total number of observations | 242344 | 2295 | 9957 |
| Number of reflections | 16541 | 205 | 909 |
| <I/σ(I)> | 19.5 | 47.5 | 2 |
| Completeness [%] | 99.8 | 99.9 | 97.6 |
| Redundancy | 14.7 | 11.2 | 11 |
| CC(1/2) | 1.000 | 0.999 | 0.845 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.3 | 291 | 0.1 M citric acid pH 4.3, 1.8 M ammonium sulfate |
