6WBH

Crystal structure of mRECK(CC4) in fusion with engineered MBP at medium resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-10
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.5419
Spacegroup name	P 21 21 2
Unit cell lengths	80.222, 110.599, 57.791
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.530 - 2.455
R-factor	0.1957
Rwork	0.194
R-free	0.21960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3set
Data reduction software	DIALS
Data scaling software	Aimless (0.7.2)
Phasing software	PHASER (2.8.1)
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.530	2.510
High resolution limit [Å]	2.450	2.450
Rmerge	0.190	0.511
Rmeas	0.208	0.562
Rpim	0.081	0.222
Number of reflections	19054	1811
<I/σ(I)>	7.4
Completeness [%]	98.3	94.52
Redundancy	6.3	5.7
CC(1/2)	0.947	0.837

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	4.6	293	0.2 M ammonium sulfate, 0.1 M sodium acetate, pH 4.6, 25% PEG 4000