6WB6

2.05 A resolution structure of transferrin 1 from Manduca sexta

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-07
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.00000
Spacegroup name	P 21 21 21
Unit cell lengths	64.493, 139.062, 146.696
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 2.050
R-factor	0.212
Rwork	0.209
R-free	0.27190
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	CRANK2
Refinement software	PHENIX (dev_3755)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.280	47.280	2.090
High resolution limit [Å]	2.050	10.650	2.050
Rmerge	0.162	0.081	0.827
Total number of observations	544369	4151	30084
Number of reflections	83612	685	4581
<I/σ(I)>	7.6	15.5	2.2
Completeness [%]	100.0	98.7	100
Redundancy	6.5	6.1	6.6
CC(1/2)	0.991	0.995	0.697

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	15% (w/v) PEG 20,000, 0.1 M Hepes