6WB4
Microbiome-derived Acarbose Kinase Mak1 Labeled with selenomethionine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-2 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-05-24 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97932 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 113.831, 123.509, 102.443 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.600 - 2.593 |
| R-factor | 0.1891 |
| Rwork | 0.186 |
| R-free | 0.25310 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.022 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | SHELX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 29.601 | 29.600 | 2.710 |
| High resolution limit [Å] | 2.590 | 8.980 | 2.590 |
| Rmerge | 0.130 | 0.057 | 1.469 |
| Rmeas | 0.134 | 0.059 | 1.507 |
| Rpim | 0.030 | 0.013 | 0.331 |
| Total number of observations | 10068 | 52206 | |
| Number of reflections | 22619 | 598 | 2645 |
| <I/σ(I)> | 16.9 | 50.1 | 2.2 |
| Completeness [%] | 99.4 | 97.4 | 95.9 |
| Redundancy | 20.2 | 16.8 | 19.7 |
| CC(1/2) | 0.999 | 0.999 | 0.802 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 1.25 M Ammonium Dihydrogen Phosphate, 0.1M Tris-HCl pH 8.3, 10 mM CaCl2, 6 mM ATP, 50 mM Acarbose |
