6WB3
Crystal structure of coiled coil region of human septin 4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 25.763, 41.463, 54.588 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.020 - 1.350 |
| R-factor | 0.2227 |
| Rwork | 0.222 |
| R-free | 0.24670 |
| Structure solution method | AB INITIO PHASING |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.870 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.31) |
| Phasing software | Arcimboldo |
| Refinement software | BUSTER (2.10.3 (6-FEB-2020)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 33.020 | 33.020 | 1.370 |
| High resolution limit [Å] | 1.350 | 7.390 | 1.350 |
| Rmerge | 0.071 | 0.067 | 0.888 |
| Rmeas | 0.074 | 0.071 | 0.926 |
| Rpim | 0.022 | 0.022 | 0.261 |
| Total number of observations | 162112 | 1036 | 8065 |
| Number of reflections | 13442 | 111 | 654 |
| <I/σ(I)> | 16.2 | 36.4 | 2.6 |
| Completeness [%] | 99.9 | 99.7 | 100 |
| Redundancy | 12.1 | 9.3 | 12.3 |
| CC(1/2) | 0.999 | 0.998 | 0.948 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.6 | 291 | 0.1 M sodium acetate pH 4.6, 0.2 M NH4SO4, 16% w/v PEG 4000 |
