6WAM

Structure of Acinetobacter baumannii Cap4 SAVED/CARF-domain containing receptor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	80
Detector technology	PIXEL
Collection date	2020-02-15
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97918
Spacegroup name	P 1 21 1
Unit cell lengths	99.718, 111.377, 173.298
Unit cell angles	90.00, 103.06, 90.00

Refinement procedure

Resolution	39.600 - 2.600
R-factor	0.1949
Rwork	0.194
R-free	0.22860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6vm6
RMSD bond length	0.004
RMSD bond angle	0.581
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.600	2.640
High resolution limit [Å]	2.600	2.600
Number of reflections	100775	5818
<I/σ(I)>	12.9
Completeness [%]	88.7
Redundancy	3.3
CC(1/2)	0.997	0.808

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	291	0.1 M Tris pH 8.5, 0.2 M LiSO4, 16% PEG-3350