6WAE
Crystal Structure of 6X-His tagged SmcR
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CMOS |
Collection date | 2018-03-15 |
Detector | RDI CMOS_8M |
Wavelength(s) | 1.00003 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 78.161, 99.323, 129.528 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 66.920 - 2.130 |
R-factor | 0.2129 |
Rwork | 0.211 |
R-free | 0.25530 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3kz9 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.703 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.15_3459) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 78.820 | 2.160 |
High resolution limit [Å] | 2.130 | 2.130 |
Rmeas | 0.147 | 1.688 |
Rpim | 0.055 | 0.684 |
Number of reflections | 57324 | 2841 |
<I/σ(I)> | 7.1 | 1.1 |
Completeness [%] | 100.0 | 100 |
Redundancy | 7.1 | 6 |
CC(1/2) | 0.999 | 0.618 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M of Lithium Sulfate, 0.1 M Imidazole buffer pH 7.6-8 and 6-10% PEG3350 |