6WAB
Crystal structure of human galectin-4 C-terminal carbohydrate recognition domain in complex with galactose derivative
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-10-22 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.698, 128.558, 45.891 |
| Unit cell angles | 90.00, 101.22, 90.00 |
Refinement procedure
| Resolution | 36.795 - 2.280 |
| Rwork | 0.197 |
| R-free | 0.26620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ym2 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.692 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.010 | 2.360 |
| High resolution limit [Å] | 2.280 | 2.280 |
| Number of reflections | 23306 | 2251 |
| <I/σ(I)> | 6.8 | |
| Completeness [%] | 98.6 | |
| Redundancy | 3.4 | |
| CC(1/2) | 0.992 | 0.766 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 1.6 M ammonium sulfate, 4% (v/v) polyethylene glycol (PEG) 400, 0.1 M HEPES pH 7.0 |
