6WAB
Crystal structure of human galectin-4 C-terminal carbohydrate recognition domain in complex with galactose derivative
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-10-22 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9537 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 45.698, 128.558, 45.891 |
Unit cell angles | 90.00, 101.22, 90.00 |
Refinement procedure
Resolution | 36.795 - 2.280 |
Rwork | 0.197 |
R-free | 0.26620 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ym2 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.692 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.010 | 2.360 |
High resolution limit [Å] | 2.280 | 2.280 |
Number of reflections | 23306 | 2251 |
<I/σ(I)> | 6.8 | |
Completeness [%] | 98.6 | |
Redundancy | 3.4 | |
CC(1/2) | 0.992 | 0.766 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 1.6 M ammonium sulfate, 4% (v/v) polyethylene glycol (PEG) 400, 0.1 M HEPES pH 7.0 |