6W9Y

De novo designed receptor transmembrane domains enhance CAR-T cytotoxicity and attenuate cytokine release

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-07
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.953737
Spacegroup name	C 2 2 21
Unit cell lengths	41.489, 64.975, 85.160
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.350 - 2.550
Rwork	0.277
R-free	0.32590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5eh6
Data reduction software	XDS (Mar 15, 2019 BUILT=20190315)
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER (2.8.2)
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.350	2.641
High resolution limit [Å]	2.550	2.550
Rmerge	0.066	0.573
Rmeas	0.075	0.640
Rpim	0.034	0.279
Number of reflections	3956	381
<I/σ(I)>	12.72	2.41
Completeness [%]	98.9	97.14
Redundancy	4.9	5
CC(1/2)	0.995	0.936

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	6	293	35 mg/ml peptide in LCP 25% w/v poly(ethylene glycol) 1500 10% v/v succinate-phosphate-glycine pH 6.0