6W9X
Crystal structure of Laccase from Thermus thermophilus HB27 with an open conformation of beta-hairpin (Average deposited dose 5.1 MGy)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-04-17 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.1950 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.521, 60.757, 76.338 |
| Unit cell angles | 90.00, 109.52, 90.00 |
Refinement procedure
| Resolution | 39.240 - 1.700 |
| R-factor | 0.2004 |
| Rwork | 0.198 |
| R-free | 0.24680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xu9 |
| Data reduction software | XDS (Version Jan 26,201) |
| Data scaling software | XSCALE (Version Jan 26,201) |
| Phasing software | PHASER (7.0.076) |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.240 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.708 | 1.266 |
| Rmeas | 0.752 | 1.350 |
| Rpim | 0.252 | 0.461 |
| Number of reflections | 51847 | 5145 |
| <I/σ(I)> | 55.41 | 5.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.7 | 8.6 |
| CC(1/2) | 0.750 | 0.539 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 5.5 | 277.15 | 20% PEG 4000, 20% 2-propanol, 100mM citrate buffer |
