6W9Q

Peptide-bound SARS-CoV-2 Nsp9 RNA-replicase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-03-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.95372
Spacegroup name	P 43 2 2
Unit cell lengths	59.011, 59.011, 85.649
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.510 - 2.050
R-factor	0.2334
Rwork	0.233
R-free	0.24590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1qz8
RMSD bond length	0.013
RMSD bond angle	1.291
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.500	2.100
High resolution limit [Å]	2.050	2.050
Rpim		0.600
Number of reflections	10055	1425
<I/σ(I)>	13.7
Completeness [%]	100.0	100
Redundancy	8.6	8.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4	298	2.2M AmSO4, 0.1M Citrate-phosphate pH4