6W9O
Crystal structure of an OTU deubiquitinase from Wolbachia pipientis wMel
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-06-28 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.96862 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 52.543, 56.641, 63.956 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.000 - 1.470 |
R-factor | 0.1646 |
Rwork | 0.163 |
R-free | 0.18980 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3c0r |
Data reduction software | XDS (Jan 10, 2014) |
Data scaling software | Aimless (0.3.6) |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 33.000 | 33.000 | 1.500 |
High resolution limit [Å] | 1.470 | 8.050 | 1.470 |
Rmerge | 0.053 | 0.024 | 0.754 |
Rmeas | 0.061 | 0.028 | 0.866 |
Rpim | 0.028 | 0.014 | 0.419 |
Total number of observations | 141445 | 925 | 6619 |
Number of reflections | 33057 | 241 | 1626 |
<I/σ(I)> | 15.4 | 49.3 | 2.7 |
Completeness [%] | 99.7 | 98.4 | 99.9 |
Redundancy | 4.3 | 3.8 | 4.1 |
CC(1/2) | 0.999 | 0.999 | 0.785 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2 M sodium acetate, 32% PEG 4K, 0.1 M Tris pH 8.5 |