6W7P
Crystal Structure Analysis of Space-grown Lysozyme - Ground experiment
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL14-1 |
Synchrotron site | SSRL |
Beamline | BL14-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-04-08 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.19499 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 78.866, 78.866, 37.155 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.600 |
R-factor | 0.1727 |
Rwork | 0.170 |
R-free | 0.22020 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5kxk |
RMSD bond length | 0.020 |
RMSD bond angle | 1.943 |
Data reduction software | XDS |
Data scaling software | SCALA (3.3.22) |
Phasing software | EPMR |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 55.767 | 39.433 | 1.690 |
High resolution limit [Å] | 1.600 | 5.060 | 1.600 |
Rmerge | 0.027 | 0.619 | |
Rmeas | 0.054 | 0.032 | 0.755 |
Rpim | 0.028 | 0.016 | 0.426 |
Total number of observations | 1752 | 6888 | |
Number of reflections | 15331 | 485 | 2288 |
<I/σ(I)> | 10.9 | 30.1 | 1.7 |
Completeness [%] | 96.2 | 82.8 | 99.6 |
Redundancy | 3.2 | 3.6 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIQUID DIFFUSION | 4.6 | 296 | PEG 6000, NaCl, Na Acetate |