6W7D

K2P2.1 (TREK-1), 10 mM K+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-07
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.03320
Spacegroup name	P 21 21 21
Unit cell lengths	67.306, 122.652, 126.127
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	14.950 - 3.500
R-factor	0.2831
Rwork	0.281
R-free	0.31100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6cq6
RMSD bond length	0.006
RMSD bond angle	0.880
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER (2.10.3 (6-FEB-2020))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.000	3.830
High resolution limit [Å]	3.500	3.500
Rmerge	0.080
Number of reflections	13736	3207
<I/σ(I)>	8
Completeness [%]	99.9
Redundancy	6.6
CC(1/2)		0.225

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	22-25% PEG400, 100mM HEPES pH=8.0, 1mM CdCl2, 200mM KCl