6W7C

K2P2.1 (TREK-1), 1 mM K+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-23
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.03321
Spacegroup name	P 21 21 21
Unit cell lengths	66.501, 123.014, 125.848
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	14.990 - 3.400
R-factor	0.2821
Rwork	0.282
R-free	0.28930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6cq6
RMSD bond length	0.005
RMSD bond angle	0.760
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER (2.10.3 (6-FEB-2020))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.700	3.670
High resolution limit [Å]	3.400	3.400
Rmerge	0.101
Number of reflections	14818	3000
<I/σ(I)>	8.6
Completeness [%]	99.9
Redundancy	6.5
CC(1/2)		0.147

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	277	22-25% PEG400, 100mM HEPES pH=8.0, 1mM CdCl2, 200mM KCl
1	VAPOR DIFFUSION, HANGING DROP	8	277	22-25% PEG400, 100mM HEPES pH=8.0, 1mM CdCl2, 200mM KCl