6W6N
K106L/A131E mutant of cytochrome P460 from Nitrosomonas sp. AL212
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-04-05 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 47.373, 80.172, 110.767 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 55.383 - 2.250 |
R-factor | 0.2596 |
Rwork | 0.258 |
R-free | 0.27750 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6amg |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX (1.14_3260) |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 110.770 | 2.330 |
High resolution limit [Å] | 2.250 | 2.250 |
Rmerge | 0.151 | 1.824 |
Number of reflections | 20467 | 1756 |
<I/σ(I)> | 7.66 | 0.99 |
Completeness [%] | 98.1 | 86.45 |
Redundancy | 6.5 | 6 |
CC(1/2) | 0.995 | 0.518 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | Protein in 50 mM MOPS pH 8.0 PEG 6000, 0.1 M sodium acetate pH 5.0, and 0.2 M NaCl |