6W6M
Single particle cryoEM structure of V. cholerae Type IV competence pilus secretin PilQ
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Energy Filter | GIF Quantum LS |
| Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 2.7 |
| Number of Particle | 252319 |
| Software | cryoSPARC,RELION,SerialEM,PHENIX,CTFFIND |
| Symmetry | C14 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
